Protein_Domain

Part:BBa_K203108:Design

Designed by: Michael Bartoschek   Group: iGEM09_Heidelberg   (2009-10-15)

Plasma membrane targeting (Myrpalm)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 86
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 46


Design Notes

Source

PCR amplification from plasmid DNA.

References

Zacharias D. A., et al.(2002): Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells, Science, Vol. 296, Issue 5569